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Canipheng

How much longer for WBS to colonize?

Question

It's been 7 days since I inoculated the WBS with spore syringe. No signs of life with the naked eye, mycelium or contamination. Kept in grow tent at 80 degrees, in the dark and undisturbed until now. Was PC for 2 hours after doing ZM wbs tek. Should I reinject more spores or wait longer? Strain is B+ from redacted (< delete post if against the rules). Thanks for looking.IMG_0182.JPG.06bcd5314438ceff15aad963db6e7827.JPG

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15 answers to this question

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Give it more time, vendor spore syringes usually take a while to see growth.

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Rye berries are too expensive where I live.

Did I mention that I'm cheap? So, I would go with the Oats.

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Alright, thanks magic. Back in the tent they go.

Should I start a LC since there's about 4 ml of spore left in the syringe? I have 3 other strains besides the B+. 

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Sounds like a plan Cue. I'm thinking about running rye berries. There's oats I can get cheap locally. Time to decide.

 

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Wait for more growth . make lc with little amount knock up stuff. Dryer jars don't germate quicky

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Oats work. So does WBS. I use both depending on what I have in my closet. Wait a little longer. Although my syringes germinate in 2-5 days  - because I dont add things to my water like lubricants and other chemicals to thicken the water, or prevent the spores from sticking to the syringe barrel/plunger. drip a little of the spore solution on your finger. I am sure you will find it is greasy, like lubricant. That is very popular with a couple of spore vendors. Those vendors syringes that are adding whatever it is to the water that makes it greasy feeling always take longer to germinate. 

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I prefer WBS, with Oats a close second.

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I got a ton of white wheat right now also. I've had it for like 3 years. I typically grab the wbs first. I actually wait for wbs to go on sale and stock up on it. But that does not mean people should not experiment with different grains and methods. Because you might find something else just works better for you than it would for me. I watch alot of people grow different ways and some of those ways, like bags, I'm just really not good at for some reason. 

Always experiment and try new things. 

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Going down this new rabbit hole will be quite an experience. I'm looking at building a glove box or maybe a flow hood. I do have an extra 6" in line fan sitting around. Been reading up on both teks. Agar confuses me a bit after reading up on it. Good thing things are slowing down around me freeing up a bit of time. So much more to learn. Thanks for the feedback.

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I had some weak spores that never colonized but I did wait 3 weeks for one and it did colonize. It was P.E that ended up fruiting something else. Like above I would wait and just start lining up the next contenders. I think sometimes you can get weak spores that just struggle the whole time.....but they surprise you too many times so patience is the game... :) good luck

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On 7/16/2017 at 0:45 PM, Mush Zombie said:

Oats work. So does WBS. I use both depending on what I have in my closet. Wait a little longer. Although my syringes germinate in 2-5 days  - because I dont add things to my water like lubricants and other chemicals to thicken the water, or prevent the spores from sticking to the syringe barrel/plunger. drip a little of the spore solution on your finger. I am sure you will find it is greasy, like lubricant. That is very popular with a couple of spore vendors. Those vendors syringes that are adding whatever it is to the water that makes it greasy feeling always take longer to germinate. 

Thats crazy, thanks for sharing.


i just did this with a leftover half syring from 101, and it is, almost if there was a bit of P.Glycol added.
Also when i PC'ed syringes the other day i had a 5 emptys from the same vendor i threw in with the ones i got from a friend working at the local hospital. Both are purported as BD brand Luer lock 12ML and looked exactly the same, except when i went to fill them, all the markings and measurements came off 5 of the barrrels onto my gloves. Im guessing there knockoffs possibly of BD brand?    

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yeah s101 tries really hard to copy my syringes. however, PG is an alcohol and will kill spores ;) sorry guys not how i make mine, try again. my aqueous solution is proprietary and many will try to copy it but never succeed :lol: heres a hint. it is not nutritive, but prevents complete freezing, while distributing the spores evenly and preventing them from sticking to the barrel and the plunger, keeping them suspended in the solution instead. 

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Is buying yours the only way to sponsor the site?

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I'm thinking my syringes are no longer viable. Shipping during the summer left them in hot mail boxes may have killed off the spores. I still have 3 syringes left that I'm still going to give a shot. I scrapped the B+ and now trying to noc the Cambodians. If the spores don't work out, I'll have to wait till the fall to reorder.

 

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