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Tanelorn

how to keep LC syringes sterile when replacing needles?

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Tanelorn

I see a lot of flame steralization and alcohol related tips, but i cant find any info on how to keep the bottom part sterile. It has to come into contact with air, but its plastic so i cant flame it. Should i dip the syringe tip and the plastic of the needle in alcohol before i connect them? Should i soak the luer lock tips in alcohol before closing the syringes for long term storage? Im afraid that contact with alcohol could harm the mycellium. I will be preparing a GLC syringe soon and i cant screw it up.

 

I have a sealed 60cc syringe and plan to pressure steralize a needle. I'll need to connect them afterwards, during which time they will need to be in contact with air. After innoculation, i plan to store the rest for later, and i will need to use a pressure steralized luer lock tip to seal it, during which they will also come into contact with air.

 

I don't have a flowhood and have had bad luck with gloveboxes, but ill follow any good advice :)

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Mush Zombie

Use all of your lc in one go that is in the syringe.

Wrap the syringe and needle in foil and pressure cook it for 15 minutes.

Keep sterile syringes on standby. 

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NineInchNails

AKA Hefe is right. Sterilize it and you don't have to worry.

Another way is to bring water to a full rolling boil for a few minutes, connect needle to syringe, suck up water, shoot it out into the sink, suck up more boiling water and repeat several times. Leave water in the syringe. Flame the needle then shoot the water out to cool the needle. Now you're good to go. Start sucking up LC.

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