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Don_Shadow

Myceliated Grain TEK

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Moonphase

Not sure I'm man enough to choke down a 6 qt. tub of colonized substrate.   :OMG:

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Don_Shadow

Medicinal mushrooms are often grown on sterile grain media like brown rice. Many mushroom products contain mycelia on finely ground or “micronized” brown rice substrate.

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Samwise
On 11/22/2019 at 5:09 AM, Moonphase said:

Not sure I'm man enough to choke down a 6 qt. tub of colonized substrate.   :OMG:

Ha not sure anyone is calling anyone to attempt that! Above four tablespoons of the P. hoogshagenii/semperviva dried mycelium grain is starting to get deep in terms of dosage...this amount is very easily consumed in tea form.

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Samwise

A friend and I sampled some Caerulescens mycelium (on rice)...it was only 6 weeks old following inoculation. We started off with 4 tablespoons of the grain gently simmered, and ingested in the form of cocoa...this first dose produced subtle effects so two hours in we had a top up, of another 3 tablespoons...so 7 tablespoons in total, but staggered. In my case, it boosted the experience a little, but it was still quite subtle...but my friend had a relatively short lasting, but immersive and powerful experience that she described as feeling quite reminiscent to her of ayahuasca, based on her prior experiences with that. Clearly our sensitivities vary! I appreciate incubation time wasn't long so this may well have impacted potency, but it seems like your experience was not a great deeper than mine. The Hoogshagenii/Semperviva seems to be a great deal more potent by weight in mycelium-grain form.. It will be interesting to compare eating grain vs tea consumption...the tea certainly seems smoother on one's system, but my feeling is that ingesting it may yield a longer lasting experience. It will be interesting to see how the oats compare.

I'm in the process of inoculating grain jars (rice, oats and rye grain) with Azurescens, Cerulescens, Mexicana, Hoogshagenii/Semperviva and Zapotecorum. My hunch is that the Mexicana won't be as potent as the Semperviva, and I'm quite intrigued about the Azurescens and Zapotecorum. I'm also making up a load of lion's mane LC, keen to experiment with this in the mix too Don. 🙂

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Viridis420

Cool read thanks for posting.

 

I've noticed how dark blue P semperviva grain spawn bruises. I haven't seen P zapotecorum or P caerulescens grain spawn bruise the way P semperviva does.. Also the sclerotia producers all bruise very noticeably.

 

 

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Samwise
50 minutes ago, Viridis420 said:

I've noticed how dark blue P semperviva grain spawn bruises. I haven't seen P zapotecorum or P caerulescens grain spawn bruise the way P semperviva does.. Also the sclerotia producers all bruise very noticeably.

Interesting. Still early days with these species but my suspicion is that the amount of blueing may be a fairly reliable indicator of potency of the mycelium grain...it isn't necessarily fool proof (as P. mexicana and P. semilanceata don't really blue on bruising and are more potent than P. cubensis gram for gram which does blue). That's interesting that the sclerotia producers blue noticeably (especially as the mushrooms...all sub-strain variants of P. mexicana according to genetic analyses...don't really blue at all). So it seems like there could be differences between the mycelium and fruit bodies of the various species in terms of alkaloidal composition and potency.

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tonz-o-funguy

where carries these strains or how can i obtain one? i have a jar of myc on brown rice is there anyway to fruit the myc?  pesa is the strain i choose to use its the most potent one i have atm. can I fruit it jar style? just put it in a sgfc or put plastic bag on top? i could even case with prezels? some wheat thins could work possibly ive be contimplating this not sure what direction even if i cased in some straw i can clean it off easly.

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El_Piraña
5 hours ago, tonz-o-funguy said:

where carries these strains or how can i obtain one? i have a jar of myc on brown rice is there anyway to fruit the myc?  pesa is the strain i choose to use its the most potent one i have atm. can I fruit it jar style? just put it in a sgfc or put plastic bag on top? i could even case with prezels? some wheat thins could work possibly ive be contimplating this not sure what direction even if i cased in some straw i can clean it off easly.

Are you sure about the pretzels and wheat thins?

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tonz-o-funguy

Hell no!! i was just guessing myself I guess im not the only one who thinks it might work lol i got a pint of fully colonized wbr that i made after reading this tek. i guess ill give it a whirl ill use half the pint bc I got some honey wheat pretzels i even make minis monos out of the plastic container they work great . i got one with eq on canary reed grass and they are looking marvelous so I like to experiment.

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Samwise

By all means experiment with P. cubensis, but I doubt the potency of its mycelium on grain while render it a worthwhile or viable species with this tek in mind. The mushrooms are easy to produce in great amounts and quickly so I don't see why the myceliated tek would be particularly advantageous...it is better suited towards the more potent species that otherwise would take a long time to fruit, be trickier to fruit and/or lower yielding. These more potent (and shamanically revered) species such as P. hoogshagenii/semperviva produce highly potent mycelium on grain which make them a much more viable species to work with with this tek in mind.

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Samwise

Sharing some information here that was shared on a thread on this tek on the DMT Nexus (thank you endlessness) that was new to me and I thought may be of interest to some here.

 

Regarding alkaloid content of fruit vs mycelium, here's a few publications I've found:

Psilocybe cubensis: 0.22-0.54% psilocybin in agar-grown mycelium (Catalfolmo & Tyler 1964) (not too different than average values for fruiting body content as referenced in Trout's SST)

Psilocybe bohemica: 0.15-0.21% psilocybin, no other alkaloids detected in mycelium grown on agar, compared to 0.54% average psilocybin + 0.01% psilocin + 0.01 baeocystin in fruiting bodies (Gartz & Muller 1989)

Psilocybe samuiensis: 0.24-0.32% psilocybin in agar-grown mycelium with no other detected alkaloids vs 0.36-0.73% psilocybin + 0.21-0.52% psilocin + 0.02-0.05% baeocystin in fruiting bodies (Gartz et al. 1994)

Panaeolus subbalteatus: 0.07% psilocybin in mycelium vs 0.08-0.70% psilocybin + 0.05-0.46% baeocystin in fruiting bodies (Gartz 1992)


Seems that it varies by species, but that in mycelium it generally only has psilocybin, and usually less than in fruiting bodies but still significant.
 

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Samwise

Yesterday I finally got round to sampling my own homegrown Stamet's neurotrophic stack, using P. hoogshagenii/Semperviva and lion's mane mycelium both grown on rice using this tek. Two of us sampled 100mg of dried, powdered Semperviva, a teaspoon of lion's mane and 100mg of niacin mixed into a fruit smoothie, to surprisingly potent effect (definitely exceeding microdosing territory!). Will lower my dose next time, as nice as it was, but intrigued to explore this more. I suspect the lion’s mane was adding something to the party, but the 10 week old Semperviva rice was markedly more potent than my previous batch that was 6 weeks old. I'm impressed, this species is definitely a dependably potent species in mycelium form, and definitely a goer as far as this tek is concerned. I definitely advocate a slightly longer incubation time going on this experience, I'll incubate future jars for 3 months.

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Fungi2b

I'm testing Tampensis with this tek atm

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Samwise

Cool, will be interested to hear you get on Fungi2b.

This is some lion's mane growing using this tek...it can be seen fruiting at the top of the jar. This is cool as apparently the mycelium and fruiting bodies of this fungus contain varying amounts or types of active compounds, so when one dries and powders the contents, you get the best of both on consumption (leaving for 2 months post-inoculation prior to harvest, and will use some jars to colonise larger growbags). I've just started incorporating this into my diet, early days but am impressed so far, I've noticed a welcome mental clarity since I've started using it. An increasing body of research to show a range of health benefits associated with this fungus, and it is one very well suited indeed to this tek.

lions mane fruiting.jpg

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MagicalCriminal

Interesting read. I stopped mush due to time constraints for harvest but myc is a different story. I still have bags of it in the fridge. I going to have to try this. My poor PC has been very lonely these last 6 months.

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tonz-o-funguy

I got some wavy cap wbr myc I was gonna throw them in the fridge after they are nicely colonized and see if they will throw any pins being thats the fruiting temp. My wavy cap culture looks like a tree branch I've never seen myc like that before!! I also did a bag of pan cyan Goliath in hope the rice will be nice lol I gotta get me a lib cap swab and do those next!!!

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MagicalCriminal

I have had a 5lb bag of ps cyan in the fridge hibernating  for about 6 months. New growth that appeared to bruise was seen a week ago. I just traded the bag for several rooted 2yo trich hybrids. 

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