Jump to content
  • Welcome Guest!

    Please register or sign in to have the complete Shroomology community experience! Become a member today, post topics, get your own profile, personal messenger and more!

Mush Zombie

Mushroom Sub-Strain Isolation

Recommended Posts

Str0be

When you isolate a substrain like this, and you end up with a dish with nothing but linear rhizomorphic mycelium, is it generally always a fruitable strain? Or can you end up isolating a strain that doesn't even produce?

Share this post


Link to post
Share on other sites
Mush Zombie

When you isolate a substrain like this, and you end up with a dish with nothing but linear rhizomorphic mycelium, is it generally always a fruitable strain? Or can you end up isolating a strain that doesn't even produce?

Yes you can wind up isolating a substrain that does not have good fruiting potential, even if its rhizomorphic.Thats why its best to start the isolation process with a clone of a mushroom from a large cluster, if a high yielding sub-strain is what you are after.
  • Like 1

Share this post


Link to post
Share on other sites
BEYOND2

thanks MZ ive been waitn for thread like this :boxxy:

Share this post


Link to post
Share on other sites
AL2O3

thanks for the post MZ. Great information

Share this post


Link to post
Share on other sites
Dozzer

Thank you MZ for posting this, it will help everyone new and old, and very easy to follow and understandPeace From The East**D**

Share this post


Link to post
Share on other sites
Str0be

A method I have been using and seems to do me justice is to induce fruiting on the plate itself. You will then see right away which sectors are good possible candidates before transfering just by the pin sets. Its a quick and effective way to get a preview of what you are possibly growing. :)MZ is right use big clusterspost-106-0-38599800-1329705241_thumb.jpgAnd you can get big flushespost-106-0-41798300-1329705365_thumb.jpgpost-106-0-88106800-1329705395_thumb.jpg

By inducing fruiting, you mean just putting your agar dishes in the same environment as you would to fruit? Basically just a room with a temp around 68 - 72?
  • Like 2

Share this post


Link to post
Share on other sites
SquidHead

Yeah if you wait long enough they will automatically. Yes you can get them to pin by dropping temps slightly and giving them some light. They remain sealed though, until I am in front of a hood and doing transfers. My theory is if they can fruit abundantly on agar then they will do even better on a nutrient rich substrate.

  • Like 1

Share this post


Link to post
Share on other sites
BEYOND2

damn that shits crazy!!!!!

Share this post


Link to post
Share on other sites
Neowulf

awesome i hope to start doing this with results from first grow

  • Like 1

Share this post


Link to post
Share on other sites
OStricher

Good info and well presented.

  • Like 1

Share this post


Link to post
Share on other sites
MrDouchebag

This couldn't have been more clear and easy to understand MZ. Thanks you!

  • Like 1

Share this post


Link to post
Share on other sites
KICKASS

Posted ImageMush Zombie, on 19 February 2012 - 04:20 PM, said:

Yes you can wind up isolating a substrain that does not have good fruiting potential, even if its rhizomorphic.

Thats why its best to start the isolation process with a clone of a mushroom from a large cluster, if a high yielding sub-strain is what you are after.

So easy to understand. I repect the scientific guys alot! But have a hard time digesting what they are saying at a novice level, and for a quick turnaround.

And I respect you for cutting out and translating this into "laymens" terms.

Thank you! Thank you! Thank you!! :)

  • Like 1

Share this post


Link to post
Share on other sites
OStricher

So the 2 lc's I just started from a single heavy rhizo is just that, a heavy rhizo myc. At this point it may be a poor producer with bad characteristics. Pretty much a 50/50 chance till I see the fruit from it. That makes sense on why a sample from a fruit with good characteristics would be preferred.

  • Like 1

Share this post


Link to post
Share on other sites
Mr.Greenthumb

awesome tek MZ im all ready doing my research so I can do this myself!!

Share this post


Link to post
Share on other sites
SlimJim

If you are using the agar jars definately. I have done that many times, not purposefully, just being a slacker. They will pin if you just leave them out somewhere like you described.

Even PE! :)

post-1-0-00489500-1329756024_thumb.jpgpost-1-0-16487000-1329756031_thumb.jpg

Really great pictures and post!

Share this post


Link to post
Share on other sites
BrutalityIsLaw

Looks like it's time to get some agar going!

  • Like 1

Share this post


Link to post
Share on other sites
Those Who Were

I got a mean rhizo growth of pe in an agar dish. my ape agar had little blue dicks that popped up in a cluster. got 4 lc's ready to make some reishi lc and ala lc. great write up MZ

Share this post


Link to post
Share on other sites
Austiclees

Dikaryotic Mycelium- www.iscid.org/encyclopedia/Dikaryotic_Cell

Link's not valid anymore. :(
  • Like 1

Share this post


Link to post
Share on other sites
 Pseudonemesis

I never thought of using widemouth jars as petri dishes... What is your opinion on isolating directly on rye? I have seen very different types of growth from the same variety growing from the same batch of grains when growing from spore... I seem to remember reading back in the day that if you grow from a single spore it won't form carpophores until the mycelium trades genetics with another genetically different mycelium culture. Do you know the terms associated with this phenomenon?

Share this post


Link to post
Share on other sites
 Pseudonemesis

in an ms culture, this whole process occurs at the inoculation point. thousands of spores germinate as monokaryons. those monokaryons are not capable of producing fruits. they mate with another monokaryon. thus dikaryotic mycelium is formed, and this is your genetically different culture (AKA sub-strain). There will be several dikaryotic cultures in a single MS culture. The objective of this thread is to show how to isolate those cultures.

That's what I thought. Do you know what the process of their "genetics swap" is called?

Share this post


Link to post
Share on other sites
Mush Zombie

That's what I thought. Do you know what the process of their "genetics swap" is called?

i am not sure of the exact terminology. The only reason why I know this is because I read a few books showing the process in pictures and descriptions. One thing that is interesting, in an MS culture the dominant substrains (which usually resemble the parent of the spores) actually convert the weaker substrains into it's substrain, until there are only a handful of dominant substrains in the culture.
  • Like 1

Share this post


Link to post
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now

  • Recently Browsing

    No registered users viewing this page.

  • Topics

  • Posts

    • OldManDan
      Pretty crappy quality pic, but, everything was white.  Started GT spores on agar here 6 days ago.  Took pic in still air box, may be contaminated now but who knows, I'll find out soon enough.   Cut into 6 pieces.  1 went into a jar of LC, 5 went into WBS jars. Going to be doing another round of WBS and 1 LC in the next week or two, whenever my PES hawaiin spores get here from different vendor.
    • Tripster727
      Beautiful! Congrats 👏
    • Tripster727
      I'm back. Nd yeah absent thoughts i got a super nice pinset going now no casing layer! on 3rd flush, also made a super clean LC. Strain is PF CLASSIC. I did make a print from the GT i doubt the sterility of it though so im not wasting any time with that yet.. Things are moving along 
    • OldManDan
      Parts per million and electrical current of the water.  Measured before you feed and after(after is measured through runoff).  You can buy a meter to test water with nutrient solution.  Although there is a K.I.S.S. method that wirks fine amd you dont need meters and a lot of water testing.  Just get Maxibloom and weigh out 7 grams per gallon of water, or use flora Nova bloom as directed.  With a ppm/EC meter you can fine tune exactly how much nutes your plants are getting for perfect dosage of the nutrients if you will.
    • Dr. Trip
      Dude myco, those things are pretty af! Quick question though, you said " PPM/ec (Going in and out) " idk what you're talking about here, PPM parts per million? of what?
×