Search the Community

Hello fellow Mycologist. Today is my first day on this site. I am learning all the things I've been doing wrong. LOL I was hoping to get some input regarding to the glove box that I made. I will pretext this post noting that the entire enclosure is completely sealed airtight. I accessed the enclosure through the end cap which is removable. Using a bilge pump blower I draw air into the enclosure, through the HEPA filter and exhausted through the fan. I keep the Clean Air circulating through any procedure I am perform in the closed box. Note , that prior to all procedures the interior of the box is sanitized using isopropyl alcohol and allowed to set for 10 minutes. Should there ever be a need to stop the air flow I plug the exhaust port from the inside. I was hoping that you experts could detect any flaws in my thinking. Any problems with my design and or is this overkill for a glove box? Your response and input is appreciated. I currently have 6 jars in the pressure cooker and one Spore syringe. I would like to inoculate in the morning. please pardon the photos this is my first post and they are out of order

HIGH QUALITY GLOVEBOX TEK CREATED BY Str0be ABOUT I recently needed to make a glovebox, but did not like any of the designs I saw, so I created my own. I did not photograph the construction all that well because I was not orignally going to make a tek out of it. But someone suggested that I should, and I figured that it could help the community. If you want / need / or are told you need a glovebox, and you want a high quality one, then follow this Tek. TOOLS REQUIRED Sharpie Ruler / Tape Measure Sharp Scissors, Dremel or any suitable tool to PRECISELY cut plastic bin Drill 1/4" Drill Bit Drill Hex Head, or Wrench to tighten bolt SHOPPING LIST (1) Large STURDY Plastic Bin / Tupperware / Tote that is Completely Clear (I Use a 106 Quart Sterlite) (2) 4" Closet Flange's (Found in Plumbing Aisle in Lowes. Get PVC, NOT Metal) (2) Closet Flange Spacers (With a Large enough hole for the Closet Flange to fit.) (8) 1/4" X 2" Bolts (8) 1/4" Wing Nuts (1) Small Tube of Clear Silicone Sealant (Red RTV Silicone does not stick to plastic) (1) Full Body Tyvek Paining Suit (1) Pair of High Quality Rubber Cleaning Gloves (Make sure they fit! Try to get ones at least 12" long.) (1) Role Gorilla or Duck Tape (1) Roll of 1/4" foam Weatherstripping THE CONSTRUCTION 1. Measure 7" from the bottom of the tub, draw several dots, and trace a straight line across the front of the tub with pencil. 2. Measure the distance from shoulder to shoulder, and add 4". 3. With the distance you calculated in #2, Center that measurement on the tub. It helps to divide the number in half, and measure that distance each way from center. Mark a small X on the line at the measurement with the sharpie. 4. Place down the Flange spacer as a Template to trace a circle. Try to center both Flange Spacers on the X Marks as even with each other as possible. It may help to line up two of the pre drilled holes with the line you drew. Trace a circle of the inside of the Flange Spacer with the sharpie. 5. With the tool of your choice, cut out the circles that you drew on your bin. A sharp pair of scissors did the trick for me, but a dremel or hot knife can do the same. Once cut, make sure the Closet Flange fits snug with the hole you cut. It should be able to go to the bottom bevel without bending the plastic of your container. 6. If your Flange and Spacers are anything like mine, I had to pre drill with a 1/4 bit to make the bolts fit properly. Do the same if they don't already fit on their own. 7. Place the Closet Flange through the hole from the inside, and place the spacer on from the outside. Hold both pieces in place, and with a 1/4" drill bit, drill in each of the 4 holes so that it goes through the plastic bin to the other piece. Place one bolt and wing nut in place to secure flange and spacer. This will be kept on temporarily to get the correct length needed on the arms. 8. Put on rubber gloves and Tyvek suit. Pull the sleeves of the tyvek suit over the gloves at a comfortable and equal height so you can still bend your arms. Using gorilla tape, tape the tyvek suit to the rubber gloves. It is easier to use smaller pieces of gorilla tape, than long strips. If you put tape in the wrong spot, you will rip the tyvek suit trying to remove it. 9. Place your arms through the closet flange holes of your bin that you set in place in step #7 and reach each arm to the furthest point that you want to be able to reach to. Draw a small line on the arms of tyvek suit when you are reaching at that furthest point. 10. Take off the suit, measure 4" evenly past the line you drew, and cut the sleeves off of the suit. 11. Remove the Closet flange and Spacer, and Put a small bead of silicone around the bottom of the Closet flange along the groove and bevel where it will be in contact with your bin. 12. Place the Closet Flanges back into the holes from the inside, so the extra 2" portions are sticking outside of your bin. 13. Place the extended gloves on the inside of the bin, and pull the sleaves through the flanges to the outside, until the line you drew is at the edge of the flange. 14. Roll the extra 4" down the closet flange as far as it will go, without moving the sharpie line away from the edge. 16. Place the spacer over the rolled down sleeve into place from the outside. Screw in all 4 bolts and wing nuts tight for each flange and spacer. 17. All along the top edge of your bin, you want to put the foam weather stripping, so that it creates a perfect seal when you put the lid on. You want no airflow at all when you work inside the glovebox. The glovebox is all complete now, but you should first wait 24 hours for the silicone to dry before working in it. I personally pull the gloves through so they are hanging out. It makes it easier to put them on before working. STERILE TECHNIQUE WITH GLOVEBOX Now that the glovebox is complete, you need to properly prepare it before you can begin work. Before each use of the glovebox, and everytime the seal is broken between uses, the following steps must be followed to properly sterilize the glove box. 1. Wipe down all inside bottom, all sides, and top with isopropyl rubbing alcohol. 2. Wipe down all sides and surfaces of items going into the glovebox As you are closing the lid and sealing it, spray Lysol into the glovebox and the air around the outside of the glovebox. 3. Wait 10 minutes before beginning any work. 4. Once you put on your gloves, rub them together with isopropyl rubbing alcohol and begin work.