Every species of mushroom has various sub-strains that differ from each other, in various ways. Some of the differences you will notice are physical, I.E., size, look, density, and another is fruiting potential. The purpose of this thread is to show how to identify these sub-strains, and isolate them, for whatever reason the cultivator has, in this example it is to attempt obtain a high yielding sub-strain, and have less competition in a bulk substrate which will allow for higher yield in its self.
It is best to do this with a clone of a mushroom, because you have a good idea of the genetics that you are after, with a clone. If you attempt this from Multi-spore, you will have no idea as to what you might get. Just luck of the draw really.
This thread is focused primarily on Psilocybe Cubensis, as it has certain characteristics of growth, that not ever species of mushroom has. However, reading this will greatly enhance your knowledge of the subject, and with a little observation you can apply anything you learn here to any species of mushroom. Psilocybe Cubensis has a couple of forms of growth. At the base of any mycelial growth that is capable of producing mushrooms, is dikaryotic mycelium. From dikaryotic mycelium comes tomentose mycelium, and rhizomorphic mycelium. Since both are dikaryotic mycelium, both are capable of producing fruits, however rhizomorphic growth is more promising to find high yielding sub-strains.
Example A (multi-spore inoculation)
Example B Rhizomorphic growth (clone)
You will notice, on agar, that there are lines and impressions in the mycelium. These barren (most of the time) lines are known as sectors (seen in example A). Sectors occur when two different dikaryotic mycelia encounter each other. So on either side of the sector are two different sub-strains. They cannot mate at this point, and basically stop growing when encountering another sub-strain. This makes it easy for the Cultivator to isolate the sub-strains that are present in the dish. In this tek, we are isolating the first linear rhizomorphic growth (as seen in the green circle in example A), to its own culture dish. The mycelium will be grown out in that dish, then transferred to grain spawn, and spawned to bulk substrate.
More on:Dikaryotic Mycelium- www.iscid.org/encyclopedia/Dikaryotic_Cell